Lys782-Cys845, with GST tag at N-terminal
33-43kDa (Reducing)
50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
·Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
1. Chrencik J E, Brooun A, Zhang H, et al. Structural basis of guanine nucleotide exchange mediated by the T-cell essential Vav1[J]. Journal of molecular biology, 2008, 380(5): 828-843.
Vav1 Guanine Nucleotide Exchange Factor (VAV1) is a member of the VAV gene family and is exclusively expressed in the hematopoietic system. It functions as a GDP/GTP nucleotide exchange factor (GEF), activating pathways that lead to actin cytoskeletal rearrangements and transcriptional alterations. VAV1 contains several characteristic structural motifs that are crucial for its role as a versatile signal transducer.
VAV1 is essential for hematopoiesis, playing a key role in the development and activation of T cells and B cells. It has also been identified as a specific binding partner for Nef proteins from HIV-1.
Expression of VAV1 is restricted to cells of hematopoietic origin, including those of the erythroid, lymphoid, and myeloid lineages. Diseases associated with VAV1 include cherubism and HIV-1 infection.
The VAV1 activity was assayed by HTRF technology. The reaction was performed by incubating the VAV1 protein and CRBN, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.
2μg (R: reducing condition, N: non-reducing condition).
The purity of VAV1 Protein Human is greater than 90% as determined by SEC-HPLC.
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