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IL-10 Recombinant Rabbit mAb (SDT-088-83)

IL-10 Recombinant Rabbit mAb (SDT-088-83)

货号: S0B0045
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    IL-10
  • 分子别名

    Interleukin-10, CSIF
  • 免疫原

    Recombinant Protein
  • 细胞定位

    Secreted
  • Accession

    P22301
  • 克隆号

    SDT-088-83
  • 抗体类型

    Rabbit mAb
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
IHC-P 1:2000
WB 1:1000
背景介绍
  • IL-10 is an anti-inflammatory cytokine that maintains the balance of the immune response, allowing the clearance of infection while minimizing damage to the host. IL-10 can also dampen the harmful immune responses elicited in autoimmunity and allergy. Many different types of cells can produce IL-10, with the major source of IL-10 varying in different tissues or during acute or chronic stages of the same infection.

  • 免疫印迹

    • WB result of IL-10 Rabbit mAb
      Primary antibody: IL-10 Rabbit mAb at 1/1000 dilution
      Lane 1: IL-10 recombinant protein lysate 10 ng
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 20 kDa
      Observed MW: 20, 37 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human spleen. Anti-IL-10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung. Anti-IL-10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-IL-10 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.