p40:Ile23-Ser328 p35:Arg23-Ser219
P40:34.7kDa (Reducing) ;P35:22.5kDa (Reducing)
＞95% by SDS-PAGE
PBS, pH7.4.
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
1. Airoldi, I. et al. (2000) Journal of Immunology 165:6880.
2. Wolf, S.F. et al. (1991) Journal of Immunology 146:3074.
3. Gearing, D. et al. (1991) Cell 66:9.
4. Merberg, D. et al. (1992) Immunology Today 13:78.
5. Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. 88:4143.
IL-12 is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit.IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity.IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The EC50 for this effect is less than 0.2ng/ml.
Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The EC50 for this effect is less than 0.2ng/ml.
2μg (R: reducing condition, N: non-reducing condition).
>95% as determined by HPLC.
Immobilized IL-12, Human(Cat. No. UA040200) at 2.0μg/mL (100μL/well) can bind IL-12 R beta 1 Fc Chimera, Human(Cat. No. UA010956) with EC50 of 0.18-0.23 μg/mL.
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