M941-H1328 (end),with N-terminal GST tag
50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij 35, 10% glycerol, pH7.5
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
The MAP3K19 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the MAP3K19 protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
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