您现在的位置： 首页   -  产品中心   -  免疫学   -   抗体
S-RMab® PD-L1 Recombinant Rabbit mAb (SDT-119-38)

S-RMab® PD-L1 Recombinant Rabbit mAb (SDT-119-38)

货号: S0B2067
价格: 700
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    PD-L1

  • 分子别名

    CD274, B7-H1

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Membrane

  • Accession

    Q9NZQ7

  • 克隆号

    SDT-119-38

  • 抗体类型

    Rabbit mAb

  • 应用

    IHC-P

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
IHC-P 1:100
背景介绍
  • Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the adaptive arm of immune systems during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. The binding of PD-L1 to the inhibitory checkpoint molecule PD-1 transmits an inhibitory signal based on interaction with phosphatases (SHP-1 or SHP-2) via Immunoreceptor Tyrosine-Based Switch Motif (ITSM). This reduces the proliferation of antigen-specific T-cells in lymph nodes, while simultaneously reducing apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells) - further mediated by a lower regulation of the gene Bcl-2. Upregulation of PD-L1 on immune cells (especially myeloid cells) can also lead to formation of an immunosuppressive environment in a highly localized manner that also allow the cancer cells to proliferate.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil.

      Anti-PD-L1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human placenta.

      Anti-PD-L1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      eat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung cancer.

      Anti-PD-L1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung cancer.

      Anti-PD-L1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      eat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervix cancer.

      Anti-PD-L1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.