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Calprotectin Recombinant rabbit mAb (SDT-049-44)

Calprotectin Recombinant rabbit mAb (SDT-049-44)

货号: S0B2060
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Calprotectin

  • 分子别名

    Protein S100-A8, Calgranulin-A, MRP-8, S100 calcium-binding protein A8

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Secreted

  • Accession

    P05109

  • 克隆号

    SDT-049-44

  • 抗体类型

    Rabbit mAb

  • 应用

    IHC-P, WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.1mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
IHC-P 1:3200
WB 1:200
IP 1:25
背景介绍
  • Calprotectin is a complex of the mammalian proteins S100A8 and S100A9. The proteins exist as homodimers but preferentially exist as S100A8/A9 heterodimers or heterotetramers (calprotectin) with antimicrobial, proinflammatory and prothrombotic properties. In the presence of calcium, calprotectin is capable of sequestering the transition metals iron, manganese and zinc via chelation. This metal sequestration affords the complex antimicrobial properties. Calprotectin is the only known antimicrobial manganese sequestration protein complex. Calprotectin comprises as much as 60% of the soluble protein content of the cytosol of a neutrophil, and it is secreted by an unknown mechanism during inflammation Faecal calprotectin has been used to detect intestinal inflammation (colitis or enteritis) and can serve as a biomarker for inflammatory bowel diseases. Blood based calprotectin (in serum and plasma) is used in diagnostics of multiple inflammatory diseases, including autoimmune diseases, like arthritis, and severe infections including sepsis.

  • 免疫印迹

    • WB result of Calprotectin Rabbit mAb                

      Primary antibody: Calprotectin Rabbit mAb at 1/200 dilution
      Lane 1: C2C12 whole cell lysate 20 µg
      Lane 2: THP-1 whole cell lysate 20 µg
      Negative control: C2C12 whole cell lysate      

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 11 kDa

  • 免疫沉淀

    • Calprotectin Rabbit mAb at 1/25 dilution (0.4 µg) immunoprecipitating Calprotectin in 0.4 mg THP-1 whole cell lysate.
      Western blot was performed on the immunoprecipitate using Calprotectin Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: THP-1 whole cell lysate 5 µg (Input)
      Lane 2: Calprotectin Rabbit mAb IP in THP-1 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in THP-1 whole cell lysate
      Predicted MW: 11 kDa
      Observed MW: 11 kDa
      （This blot was developed with high sensitivity substrate）

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human spleen. Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver.

      Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervix cancer.

      Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung cancer.

      Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat spleen.

      Anti-Synaptophysin antibody was used at 1/3200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.