您现在的位置： 首页   -  产品中心   -  免疫学   -   抗体
Beta 2 microglobulin Recombinant rabbit mAb (SDT-096-100)

Beta 2 microglobulin Recombinant rabbit mAb (SDT-096-100)

货号: S0B0014
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Beta 2 microglobulin

  • 分子别名

    Beta-2-microglobulin form pI 5.3, B2M

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Secreted, Cell Surface

  • Accession

    P61769

  • 克隆号

    SDT-096-100

  • 抗体类型

    Rabbit mAb

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu, Ms

  • 预测反应种属
    (反应种属缩写表)

    Or

  • 纯化方式

    Protein A

  • 浓度

    0.25mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
IHC-P 1:1000
WB 1:500
背景介绍
  • β2 microglobulin (B2M) is a component of MHC class I molecules. MHC class I molecules have α1, α2, and α3 proteins which are present on all nucleated cells (excluding red blood cells). In humans, the β2 microglobulin protein is encoded by the B2M gene. An additional function is association with the HFE protein, together regulating the expression of hepcidin in the liver which targets the iron transporter ferroportin on the basolateral membrane of enterocytes and cell membrane of macrophages for degradation resulting in decreased iron uptake from food and decreased iron release from recycled red blood cells in the MPS (mononuclear phagocyte system) respectively. Loss of this function causes iron excess and hemochromatosis. In patients on long-term hemodialysis, it can aggregate into amyloid fibers that deposit in joint spaces, a disease, known as dialysis-related amyloidosis. Levels of β2 microglobulin can be elevated in multiple myeloma and lymphoma, though in these cases primary amyloidosis (amyloid light chain) and secondary amyloidosis (amyloid associated protein) are more common.[clarification needed] The normal value of β2 microglobulin is < 2 mg/L.

  • 免疫印迹

    • WB result of beta 2 microglobulin Rabbit mAb                 Primary antibody: beta 2 microglobulin Rabbit mAb at 1/500 dilution
      Lane 1: Daudi whole cell lysate 20 µg
      Lane 2: Hela whole cell lysate 20 µg
      Lane 3: Jurkat whole cell lysate 20 µg
      Lane 4: HepG2 whole cell lysate 20 µg
      Negative control: Daudi whole cell lysate        Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 12 kDa
      Observed MW: 12 kDa
      Exposure time: 180s

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human skin.

      Anti-Beta 2 microglobulin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer.

      Anti-Beta 2 microglobulin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-Beta 2 microglobulin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver.

      Anti-Beta 2 microglobulin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.