PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IF | 1:500 |
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD45 antibody (S0B0228) was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (FITC Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human colon. Anti-CD45 antibody (S0B0228) was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (FITC Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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