PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:50 |
DKK-1 protein is a secreted protein belonging to the DKK protein family that plays crucial roles in various biological processes. DKK-1 serves as an effective antagonist of canonical Wnt signaling, inhibiting the interaction between LRP5/6 and Wnt. It forms a ternary complex with KREMEN, leading to the internalization of LRP5/6. Additionally, DKK-1 has pro-apoptotic functions independent of Wnt antagonism through KREMEN1 and exhibits anti-apoptotic activities. This protein is associated with limb development, ensuring normal limb patterning by regulating Wnt signaling. DKK-1 protein has potential applications in diagnostic testing for osteoporosis, bone tumors, bone metabolic diseases, and cardiovascular disease risk assessment. For instance, measuring DKK-1 levels in serum or urine can aid in the diagnosis and assessment of osteoporosis severity and fracture risk. In bone tumor diagnosis, elevated DKK-1 expression may provide auxiliary evidence and be used for prognostic evaluation. Additionally, DKK-1 is associated with various bone metabolic diseases such as rheumatoid arthritis and hyperparathyroidism, where it serves as an important biomarker for diagnosis and monitoring.
WB result of DKK-1 Recombinant Rabbit mAb
Primary antibody: DKK-1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Lane 2: Caco-2 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Negative control: U-2 OS whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 35~45 kDa
DKK-1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating DKK-1 in 0.4 mg Caco-2 whole cell lysate.
Western blot was performed on the immunoprecipitate using DKK-1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Caco-2 whole cell lysate 20 µg (Input)
Lane 2: DKK-1 Rabbit mAb IP in Caco-2 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Caco-2 whole cell lysate
Predicted MW: 29 kDa
Observed MW: 35~45 kDa
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