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CD20 Flag&His Tag Protein, Human (Detergent)

CD20 Flag&His Tag Protein, Human (Detergent)

货号: UA060019
价格: 7160
规格: 25μg
产品规格

  • 物种

    Human

  • 分子别名

    B-lymphocyte surface antigen B1，MS4A1，B-lymphocyte antigen CD20，Membrane Spanning 4-Domains A1，Leukocyte Surface Antigen Leu-16，CD20 Antigen，CD20，Bp35，Membrane-Spanning 4-Domains Subfamily A Member 1，CD20 Receptor，LEU-16，CVID5，MS4A2，B1，S7，Antigens， CD20，Membrane-Spanning 4-Domains， Subfamily A， Member 1，B-Lymphocyte Cell-Surface Antigen B1

  • Accession

    P11836

  • 浓度

    0.798mg/ml by Nanodrop

  • 表达序列

    Full length, with Flag tag at N-Terminal, and His tag at C-Terminal

  • 表达宿主

    Baculovirus-InsectCells

  • 分子量

    35.3 kDa

  • 纯度

    ＞90% by SDS-PAGE & ＞85% by SEC-HPLC

  • 内毒素含量

    <0.1EU/μg

  • 活性

    1.Immobilized CD20 Flag&His tag, Human (Cat. No. UA060019) at 5μg/mL (100μL/well) can bind S-RMab® CD20 Recombinant Rabbit mAb (SDT-R133) (Cat. No. S0B2158) with EC50 of 1.90-2.94ng/mL. 2.Immobilized S-RMab® CD20 Recombinant Rabbit mAb (SDT-R133) (Cat. No. S0B2158) at 1μg/mL (100μL/well) can bind CD20 Flag&His tag, Human (Cat. No. UA060019) with EC50 of 8.12-9.46ng/mL.

  • 标记

    Unconjugated

  • 标签蛋白&酶切位点

    N/A

  • 标签

    Flag Tag, His Tag

  • 性状

    Liquid with detergent

  • 缓冲体系

    25 mM Tris, pH 7.5, 150 mM NaCl, 0. 02 % DDM.

  • 储存条件

    ·6 months from date of receipt, -60 to -80 °C as supplied.
    ·about 3 days in 2 to 8 °C.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. Rougé L, Chiang N, Steffek M, et al. Structure of CD20 in complex with the therapeutic monoclonal antibody rituximab[J]. Science, 2020, 367(6483): 1224-1230.

  • 稀释度

背景介绍

  • CD20 is a 33-37 kDa non-glycosylated protein present on the surface of normal and malignant B lymphocytes and is a member of the MS4A (membrane-spanning 4-domain family A) protein family. The CD20 protein comprises four hydrophobic transmembrane domains, one intracellular domain, and two extracellular domains (a large loop and a small loop), with both the N- and C-termini located within the cytosol. CD20 is associated with several key molecules, including the major histocompatibility complex class II (MHCII), CD40 molecule, B-cell receptor (BCR), and the C-terminal src kinase-binding protein (CBP). CBP interacts with Src kinases such as LYN, FYN, and LCK.
    A deficiency in CD20 has been shown to result in a reduced number of circulating memory B cells, decreased isotype switching of immunoglobulins (Ig), and lower IgG antibody levels. It is also linked to various pathophysiological diseases and disorders.
    CD20 plays a crucial role in multiple physiological processes and is thus a promising therapeutic target. The CD20 protein product has the potential to enhance drug discovery efforts in related fields.

  • 电泳JSON

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱JSON(SEC-HPLC)

    • The purity of CD20 His&Flag tag Protein, Human (Detergent) is greater than 85% as determined by SEC-HPLC.

  • ELISA

    • Immobilized S-RMab® CD20 Recombinant Rabbit mAb (SDT-R133) (Cat. No. S0B2158) at 1μg/mL (100μL/well) can bind CD20 Flag&His tag, Human (Cat. No. UA060019) with EC50 of 8.12-9.46ng/mL.

    • Immobilized CD20 Flag&His tag, Human (Cat. No. UA060019) at 5μg/mL (100μL/well) can bind S-RMab® CD20 Recombinant Rabbit mAb (SDT-R133) (Cat. No. S0B2158) with EC50 of 1.90-2.94ng/mL.