12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| ICC | 1:200 |
| IF | 1:200 |
Antigen Ki-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore, it is associated with ribosomal RNA transcription. Ki-67 was present in the nuclei of cells in the G1, S, and G2 phases of the cell division cycle as well as in mitosis. Quiescent or resting cells in the G0 phase did not express the Ki-67 antigen. Ki-67 protein was used as diagnostic tools in different types of neoplasms because it was present in all proliferating cells (normal and tumor cells) and was an excellent operational marker to determine the growth fraction of a given cell population.
ICC shows positive staining in HeLa cells. Anti- Ki67 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
IF shows positive staining in paraffin-embedded human gastric cancer. Anti- Ki67 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human tonsil. Anti- Ki67 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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