12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IF | 1:200 |
Cytokeratin 19 fragment (CYFRA 21-1) is a fragment of cytokeratin 19 that is typically associated with epithelial cell cancers, including NSCLC, and is typically associated with the SQLC type. Since cytokeratins are structural proteins of keratin-containing intermediate filaments found in the epithelial cells, their degradation produces soluble fragments which are measurable in the blood of lung cancer patients as a tumor marker. Other examples of cytokeratin fragments include tissue polypeptide antigen and tissue polypeptide-specific antigen. CYFRA 21-1 is known to be correlated with disease response and the prognosis of lung cancer but cannot be used to differentiate cancer patients from patients with respiratory diseases.
IF shows positive staining in paraffin-embedded human breast cancer. Anti-Cytokeratin 19 fragment (CY211) (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Cytokeratin 19 fragment (CY211) (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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