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Alexa Fluor® 647 Mouse Anti-Human CD235a/b Antibody (S-R403)

Alexa Fluor® 647 Mouse Anti-Human CD235a/b Antibody (S-R403)

货号: S0B1562
价格: 650
规格: 20T
介绍: -
其他: -
产品规格
  • 宿主来源

    Mouse
  • 抗原名称

    CD235a/b
  • 分子别名

    Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, GYPA, Glycophorin-B, PAS-3, SS-active sialoglycoprotein, Sialoglycoprotein delta, GYPB
  • 细胞定位

    Cell membrane
  • Accession

    P02724, P06028
  • 克隆号

    S-R403
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG2b,k
  • 应用

    FCM
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein A
  • 浓度

    20μg/ml
  • 标记

    Alexa Fluor® 647
  • 性状

    Liquid
  • 缓冲体系

    PBS, 0.1% BSA, 0.01% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
稀释度
应用 稀释度
FCM 5 μl per million cells in 100μl volume
背景介绍
  • Glycophorin A (GYPA) and B (GYPB; this protein) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups respectively. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include the Miltenberger (Mi) complex and several isoforms of Stones (Sta); also Dantu, Sat, Henshaw (He or MNS6), Mg and deletion variants Ena, S-s-U- and Mk. Most of these are the result of gene recombinations between GYPA and GYPB.

  • 流式分析

    • Flow cytometric analysis of Human CD235a/b expression on K562. Cells from the K562 (Human chronic myelogenous leukemia lymphoblast, Right) or MOLT-4 (Human lymphoblastic leukemia T lymphoblast, Left) was stained with Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Black line histogram) and SDT Alexa Fluor® 647 Mouse Anti-Human CD235a/b Antibody (Red line histogram) at 0.1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
  • 实验方案