12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000-1:5000 |
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Tubulin α- and β-subunits have molecular weights of ~ 50 kDa and are 36%–42% identical and 63% homologous. Both tubulin subunits bind guanine nucleotides. The binding to α-tubulin at the N-site is nonexchangeable, while the binding to β-tubulin at the E-site is exchangeable. Nucleotide in microtubules does not exchange with the solution, except for terminal subunits at microtubule ends.
WB result of α-tubulin Recombinant Mouse mAb (HRP Conjugate)
Primary antibody: α-tubulin Recombinant Mouse mAb (HRP Conjugate) at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: C6 whole cell lysate 20 µg
Predicted MW: 52 kDa
Observed MW: 55 kDa
WB result of α-tubulin Recombinant Mouse mAb (HRP Conjugate)
Primary antibody: Pα-tubulin Recombinant Mouse mAb (HRP Conjugate) at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: C6 whole cell lysate 20 µg
Predicted MW: 52 kDa
Observed MW: 55 kDa
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