PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
应用 | 稀释度 |
---|---|
mIHC | 1:200-1:2000 |
B7-H4, CD4, CD66b, CD163, CD8α Antibody Panel contains 5 recombinant monoclonal antibodies against B7-H4, CD4, CD66b, CD163 and CD8α. They are provided as a sampler panel. Panel contains: - B7-H3 Recombinant Rabbit mAb (SDT-249-26) S0B2244 - CD4 Recombinant Rabbit mAb (SDT-R163) S0B2179 - CD66b Recombinant Rabbit mAb (S-766-55) S0B0521 - S-RMab® CD163 Recombinant Rabbit mAb (SDT-R164) S0B2194 - CD8α Recombinant Mouse mAb (SDT-126-8) S0B2148
mIHC shows positive staining in paraffin-embedded human tonsil (A). Anti- B7-H3 Rabbit mAb (S0B2224) was used at 1/1000 dilution (Red, B), Anti-CD4 Rabbit mAb (S0B2179) was used at 1/200 dilution (Yellow, C), Anti-CD66b Rabbit mAb (S0B0521) was used at 1/1000 dilution (Orange, D), Anti- CD163 Rabbit mAb (S0B2194) was used at 1/2000 dilution (magenta, E), and Anti- CD8α Mouse mAb (S0B2148) was used at 1/1000 dilution (Green, F), followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with mIHC staining protocol.