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Mouse TNF-α OneStep ELISA Kit

Mouse TNF-α OneStep ELISA Kit

货号: S0C3023
价格: 3200
规格: 1x96T
介绍: -
其他: -
产品规格
  • 抗原名称

    TNF-α
  • 免疫原

    Recombinant Protein
  • 抗体类型

    Recombinant mAb
  • 反应种属 ?

    Ms
  • 纯化方式

    Protein A
  • 储存条件

    2 to 8 °C as supplied.
  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC-P", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
试剂盒参数
    • Precision

      Intra-assay: 3.8%;

      Inter-assay: 5.8%

    • Sample type

      Cell culture supernatant, Serum, Plasm
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      0.095 ng/ml
    • Range

      0.156 ng/mL – 10 ng/mL
    • Recovery

      Cell culture supernatant: 87%
      Serum: 106%
      Plasm: 105%
    • Assay time

      60 minutes
    • Species reactivity

      Mouse
    • CROSS REACTIVITY

      /
    • INTERFERENCE

      /
    • NIBSC convert

      /
背景介绍
  • Tumor necrosis factor α (TNF alpha) is an effective pro-inflammatory cytokine, which can kill and inhibit tumor cells. It is mainly produced by activated macrophages and can also be secreted by other types of cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils and neuronal tissues. The members of TNF alpha family exert their cellular effect through two distinct surface receptors of the TNF receptor family, TNFR1and TNFR2. The pleiotropic biological effects of TNF can be attributed to its ability to simultaneously activate multiple signaling pathways in cells. TNF-induced NF-κB activation promotes the growth, invasion and metastasis of cancer cells.

  • ELISA

    • Standard curve
      Example of Mouse TNF-α standard curve in Assay Diluent #1.

    • Linearity

      The concentrations of TNF-α were measured and interpolated from the target standard curves and corrected for sample dilution.

      Sample is undiluted samples are as follows:  RAW264.7 mouse monocyte/macrophage cells stimulated with 5ug/ml LPS and 100ng/mL rmIL-10 for 7days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 13.19 ng/mL in stimulated RAW264.7 supernatant.

    • Protocol Diagram