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Biotin Kras[G12A] Protein, Human

Biotin Kras[G12A] Protein, Human

货号: UA080271
价格: 3970
规格: 100μg
介绍: -
其他: -
产品规格
  • 物种

    Human
  • Accession

    P01116-2
  • 表达序列

    M1-K169

  • 表达宿主

    E.coli
  • 分子量

    23.5 kDa

  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <1EU/μg
  • 活性

    HTRF assay
  • 标记

    Unconjugated
  • 标签

    Avi Tag, His Tag
  • 性状

    Liquid
  • 缓冲体系

    50 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5
  • 储存条件

    Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.

  • 稀释度

  • 生物活性JSON

    • The Biotin-KRAS[G12A] activity was detected using HTRF technology. The reaction was performed by incubating the Biotin-KRAS[G12A] protein, GTP and SOS1 at 25℃ for 60 min, adding cRAF at 25℃ for 15 min and adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.

  • 电泳JSON

    • M   MW Standard

      1   1 μg Biotin-Kras[G12A]

      2   3 μg Biotin-Kras[G12A]