40-50 kDa (Reducing)
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Supuran CT. Carbonic anhydrases: novel therapeutic applications for inhibitors and activators. Nat Rev Drug Discov 2008; 7:168-81.
McDonald PC, Swayampakula M, Dedhar S. Coordinated regulation of metabolic transporters and migration/invasion by carbonic anhydrase IX. Metabolites 2018;8: E20.
The CAIX works as a dimeric molecule composed of two monomeric proteins liked with a disulfide bond between cysteine residues of the two CAIX monomers, while the structure of CAXII is stabilized by 19 hydrogen-bonded interactions in the dimer interface. Carbonic anhydrases IX and CAXII (CAIX/CAXII) are transmembrane zinc metalloproteins that catalyze a very basic but crucial physiological reaction: the conversion of carbon dioxide into bicarbonate with a release of the proton. CAIX functions with bicarbonate transporters (including electroneutral bicarbonate transporter NBCn1 (SLC4A7)) to bring in HCO3- produced in a CAIX-mediated hydration reaction of CO2, and amino acid transporters (including the large neutral amino acid transporter 1 (LAT1) and CD98 heavy chain (CD98hc)) to transport in essential amino acids and glutamine (via interaction with glutamine transporter SLC1A5 and sodium-coupled neutral amino acid transporter 2 (SNAT2) facilitating the uptake of amino acid by SLC1A5), which cancer cells employ as an alternative metabolic energy source and biosynthetic precursor. CAIX also creates unique interactions with collagen- and laminin-binding integrins at the leading edge of migrating cells.
1μg (R: reducing condition, N: non-reducing condition).
The purity of Carbonic Anhydrase IX/CA9 His Tag, Cynomolgus is more than 95% determined by SEC-HPLC.
Immobilized Carbonic Anhydrase IX/CA9 His Tag, Cynomolgus (Cat. No. UA010916) at 2.0μg/mL (100μL/well) can bind Carbonic Anhydrase 9 Recombinant Rabbit mAb with EC50 of 1.13-1.30 ng/ mL.
您现在的位置：