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LIF Protein, Rat

LIF Protein, Rat

货号: UA040244
价格: 1200
规格: 10μg
介绍: -
其他: -
产品规格

  • 物种

    Rat

  • 分子别名

    Leukemia inhibitory factor; Differentiation-stimulating factor; lif; D factor

  • Accession

    P17777

  • 表达序列

    Ser23-Phe202

  • 表达宿主

    E.coli

  • 分子量

    18kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE and HPLC

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 < 10ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM Tris, 200mM NaCl, 1m DTT, pH8.0

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. Ryffel B. et al. (1993) International Review of Experimental Pathology. 34: 69-72.

  • 稀释度

背景介绍

  • The leukemia inhibitory factor (LIF) belongs to the interleukin-6 (IL-6) cytokine family, which is involved in various biological processes including inflammation, immune responses, and embryonic development. Leukemia inhibitory factor is a glycoprotein growth and differentiation factor with pleiotropic activity. LIF has potent effects on the hematopoietic system, including megakaryocyte progenitor cells. In addition, LIF has bone regeneration activity, induces cachexia and acute-phase response in hepatocytes, and inhibits adipogenesis, to mention the more important activities. In vivo LIF treatment in monkeys and rodents was followed by signs of general toxicity, cachexia, acute-phase reaction, and stimulation of hematopoiesis. The safety margin for possible therapeutic effects on hematopoiesis seems to be very narrow.

  • 生物活性JSON

    • Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells. The EC50 for this effect is less than 10ng/ml.

  • 电泳JSON

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱JSON(SEC-HPLC)

    • >95% as determined by SEC-HPLC.