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Human IgG3 F(c) Recombinant Rabbit mAb (S-459-75)

Human IgG3 F(c) Recombinant Rabbit mAb (S-459-75)

货号: S0B0312
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Human IgG3 F(c)

  • 分子别名

    Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Secreted

  • Accession

    P01860

  • 克隆号

    S-459-75

  • 抗体类型

    Recombinant mAb

  • 应用

    IHC-P, ELISA, WB

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC 1:2000
背景介绍

  • The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].

  • 免疫印迹

    • WB result of Human IgG3 F(c) Rabbit mAb
      Primary antibody: Human IgG3 F(c) Rabbit mAb at 1/1000 dilution
      Lane 1: Human serum lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 60 kDa
      Observed MW: 60 kDa

    • WB result of Human IgG3 F(c) Rabbit mAb
      Primary antibody: Human IgG3 F(c) Rabbit mAb at 1/1000 dilution
      Lane 1: IgG1 F(c) recombinant protein lysate 10 ng
      Lane 2: IgG2 F(c) recombinant protein lysate 10 ng
      Lane 3: IgG3 F(c) recombinant protein lysate 10 ng
      Lane 4: IgG4 F(c) recombinant protein lysate 10 ng
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: IgG1 F(c)、IgG2 F(c)、IgG4 F(c): 27kDa; IgG3 F(c): 33kDa
      Observed MW: IgG3 F(c): 40kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG3 F(c) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human spleen. Anti-Human IgG3 F(c) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-Human IgG3 F(c) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Human IgG3 F(c) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.