12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000-1:10000 |
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues.
WB result of His-tag Rabbit mAb
Primary antibody : His-tag Rabbit mAb at 1/1000 dilution
Lane 1: supernantant of Expi293F transfected with His tagged CGB3 10 μg
Lane 2: purified recombinant His tagged IFN-α1 10 ng
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: His tagged CGB3: 17 kDa;
His tagged IFN-α1: 21kDa
Observed MW: His tagged CGB3: 34 kDa;
His tagged IFN-α1: 21kDa
Exposure time: 60s
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