| 应用 | 稀释度 |
|---|---|
| IP | 1:25 |
| IHC-P | 1:2000 |
| ICC | 1:100 |
| WB | 1:1000 |
| FCM | 1:500 |
CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B lymphocytes and natural killer cells. CD38 functions in cell adhesion, signal transduction and calcium signaling. The loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications including social amnesia possibly related to autism. The CD38 protein is a marker of cell activation. It has been connected to HIV infection, leukemias, myelomas, solid tumors, type II diabetes mellitus and bone metabolism, as well as some genetically determined conditions.
WB result of CD38 Rabbit mAb
Primary antibody: CD38 Rabbit mAb at 1/1000 dilution
Lane 1: HEK293 whole cell lysate 20 µg
Lane 2: Daudi whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: Ramos whole cell lysate 20 µg
Negative control: Hela whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 40 kDa
Exposure time: Lane 1, Lane 2, Lane 4: 20s ; Lane 3: 120s
Flow cytometric analysis of HeLa (left) / Ramos (right) cells labelling CD38 antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: Hela
CD38 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating CD38 in 0.4mg Daudi whole cell lysate. Western blot was performed on the immunoprecipitate using CD38 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: Daudi whole cell lysate 10µg (input)
Lane 2: CD38 Rabbit mAb IP in Daudi whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Daudi whole cell lysate
Predicted MW: 34 kDa
Observed MW: 40 kDa
Exposure time: 4s
IHC shows positive staining in paraffin-embedded human tonsil.
Anti-CD38 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen.
Anti-CD38 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon.
Anti-CD38 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer.
Anti-CD38 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer.
Anti-CD38 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human skeletal muscle(negative tissue).
Anti-CD38 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows membrane staining in Jurkat cells.
Anti-CD38 antibody was used at 1/100 dilution and incubated overnight at 4°C.
Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution.
The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100.
Nuclei were counterstained with DAPI.
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