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Human LCK, His tag

Human LCK, His tag

货号: S0A6011
价格: 500
规格: 25μg
介绍: -
其他: -
产品规格
  • 物种

    Human
  • Accession

    P06239
  • 表达序列

    Protein sequence(P06239, Asn64-Thr226, with C-10*His)
    NLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCTRLSRPCQTGGGGSHHHHHHHHHH

  • 表达宿主

    HEK293
  • 分子量

    Theoretical: 20.1kDa Actual: 24-30kDa
  • 纯度

    >90% by SDS-PAGE

  • 内毒素含量

    <1EU/μg
  • 标记

    Unconjugated
  • 标签

    His Tag
  • 性状

    Lyophilized Powder
  • 缓冲体系

    Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.
  • 溶解方法

    Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
  • 储存条件

    • 12 months from date of receipt, -20 ℃ to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.  
    • Please avoid repeated freeze-thaw cycles. 
  • 稀释度

背景介绍
  • Lymphocyte-specific protein tyrosine kinase (LCK) is a member of the Src family of protein tyrosine kinases (PTKs). It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK has emerged as one of the key molecules regulating T-cell functions. Studies using LCK knock-out mice or LCK-deficient T-cell lines have shown that LCK regulates the initiation of TCR signaling, T-cell development, and T-cell homeostasis. Because of the crucial role of LCK in T-cell responses, strategies have been employed to redirect LCK activity to improve the efficacy of chimeric antigen receptors (CARs) and to potentiate T-cell responses in cancer immunotherapy. In addition to the well-studied role of LCK in T cells, evidence has been accumulated suggesting that LCK is also expressed in the brain and in tumor cells, where it actively takes part in signaling processes regulating cellular functions like proliferation, survival and memory. Therefore, LCK has emerged as a novel druggable target molecule for the treatment of cancer and neuronal diseases. 

  • 电泳JSON

    • 2μg (R: reducing conditions)