PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| ELISA | 1:5000-1:30000 |
| WB | 1:5000-1:20000 |
OneStep polymer HRP is the enzyme polymer conjugated with secondary antibodies. The labeled polymer does not contain avidin or biotin, thus, non-specific staining caused by endogenous biotin is completely eliminated. It utilizes a novel controlled and compact enzyme polymerization technology to achieve higher sensitivity without background. The secondary antibody is intended for use with primary antibodies from rabbit for localization and identification of specific antigens on a slide mounted tissue sections by light microscopy.
S0B4002 or secondary antibody from competitor was used at 1/10000 dilution in ELISA
Recommend Dilution:1:5000-1:30000
Primary antibody :β-Tubulin (S0B0004) Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: SK-BR-3 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody:
Goat Anti-Rabbit IgG, (H+L), HRP (S0B4002)
Molecular weight: 50 kDa
Recommend Dilution:1:5000-1:20000
Primary antibody :β-Tubulin (S0B0004) Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: SK-BR-3 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat anti-Rabbit IgG HRP from conpetitor at 1/10000 dilution
Molecular weight: 50 kDa
Recommend Dilution:1:5000-1:20000
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