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Bcl-2 Recombinant Rabbit mAb (SDT-005-24)

Bcl-2 Recombinant Rabbit mAb (SDT-005-24)

货号: S0B2001
价格: 260
规格: 10μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Bcl-2
  • 免疫原

    Synthetic Peptide
  • Accession

    P10415
  • 克隆号

    SDT-005-24
  • 抗体类型

    Rabbit mAb
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein A
  • 研究领域

    Apoptosis
  • 浓度

    1 mg/ml
  • 分子量

    26kDa

  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
IHC-P 1:500-1:2000
WB 1:5000
背景介绍
  • Bcl-2 (B-cell lymphoma 2) is the founding member of the Bcl-2 family of regulator proteins that regulate cell death (apoptosis), by either inhibiting (anti-apoptotic) or inducing (pro-apoptotic) apoptosis. It was the first apoptosis regulator identified in any organism. Bcl-2 is localized to the outer membrane of mitochondria, where it plays an important role in promoting cellular survival and inhibiting the actions of pro-apoptotic proteins. Damage to the Bcl-2 gene has been identified as a cause of a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer, and a possible cause of schizophrenia and autoimmunity. It is also a cause of resistance to cancer treatments.

  • 免疫印迹

    • WB result of anti-Bcl-2 antibody                

      Primary antibody : Anti-Bcl2 antibody at 1/5000 dilution
      Lane 1 : Daudi whole cell lysate 20 µg
      Lane 2 : Jurkat whole cell lysate 20 µg
      Lane 3 : THP-1 whole cell lysate 20 µg
      Lane 4 : MCF7 whole cell lysate 20 µg
      Lane 5 : Hela whole cell lysate 20 µg
      Negative control: Daudi whole cell lysate                                   

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution


      Predicted MW: 26 kDa
      Observed MW: 26 kDa
      econds

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil.

      Anti-Bcl2 antibody was used at 1/250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.              

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.