PBS, 0.1% BSA, 0.01% Proclin 300
| 应用 | 稀释度 |
|---|---|
| FCM | 5 μl per million cells in 100μl volume |
CD8A encodes the CD8 alpha chain of the αβT cells, proposed as a quantifiable indicator for CD8+ CTL recruitment or activity assessments and a robust biomarker for responses to anti-PD-1/PD-L1 therapy. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Multiparameter flow cytometric analysis of Human CD8α expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Phycoerythrin Mouse Anti-Human CD4 antibody and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Left panel) or SDT Alexa Fluor® 647 Mouse Anti-Human CD8α Antibody (Right panel) at 1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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