| 应用 | 稀释度 |
|---|---|
| FCM | 5 μl per million cells in 100μl volume |
The Cluster of differentiation 80 (also CD80 and B7-1) is a B7, type I membrane protein in the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is closely related to CD86, another B7 protein (B7-2), and often works in tandem. Both CD80 and CD86 interact with costimulatory receptors CD28, CTLA-4 (CD152) and the p75 neurotrophin receptor [PMID: 7545666].
Flow cytometric analysis of Human CD80 expression on Raji. Cells from the Raji (Human Burkitt's lymphoma B lymphocyte, Right) or 293T (Human embryonic kidney epithelial cell, Left) was stained with PE-Cy7 Rabbit IgG Isotype Control (Black line histogram) and SDT PE-Cy7 Rabbit Anti-Human CD80 Antibody (Red line histogram) at 0.1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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