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c-Rel Recombinant Rabbit mAb (S-1150-52)

c-Rel Recombinant Rabbit mAb (S-1150-52)

货号: S0B0779
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    c-Rel
  • 分子别名

    Proto-oncogene c-Rel
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm, Membrane
  • Accession

    P15307
  • 克隆号

    S-1150-52
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    ICFCM, IHC-P, ICC, WB, IP
  • 反应种属 ?

    Ms, Rt
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:200
ICC 1:50
ICFCM 1:50
IP 1:50
背景介绍
  • c-Rel is a member of the NF-κB family, predominantly expressed in lymphocytes and myeloid cells. As a transcription factor, it regulates the proinflammatory polarization of myeloid cells and modulates the antitumor immune response. Research has found that c-Rel serves as an important checkpoint for the immunosuppressive function of myeloid-derived suppressor cells (MDSCs). MDSCs hinder the normal function of immune cells in a tumor environment, promoting tumor immune evasion. c-Rel controls the polarization of myeloid cells in tumors, and specific inhibition of c-Rel significantly inhibits tumor growth. In c-Rel-deficient (Rel–/–) mice, melanoma and lymphoma tumor growth was significantly suppressed, with a 80% reduction in tumor size and body weight compared to controls. Inhibiting c-Rel through small molecule inhibitors or conditional knockout in MDSCs can promote the body's antitumor immune response. Combining c-Rel inhibitors with PD-1 functional blocking antibodies can further enhance the activation of the body's antitumor immune response.

  • 免疫印迹

    • WB result of c-Rel Recombinant Rabbit mAb
      Primary antibody: c-Rel Recombinant mAb at 1/1000 dilution
      Lane 1: RAW264.7 whole cell lysate 20 µg
      Lane 2: Neuro-2a whole cell lysate 20 µg
      Lane 3: mouse spleen lysate 20 µg
      Lane 4: mouse thymus lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 65 kDa
      Observed MW: 70 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) labelling c-Rel antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • c-Rel Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating c-Rel in 0.4 mg Neuro-2a whole cell lysate.
      Western blot was performed on the immunoprecipitate using c-Rel Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: Neuro-2a whole cell lysate 20 µg (Input)
      Lane 2: c-Rel Rabbit mAb IP in Neuro-2a whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in Neuro-2a whole cell lysate
      Predicted MW: 65 kDa 
      Observed MW: 70 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti- c-Rel antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse testis. Anti- c-Rel antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat spleen. Anti- c-Rel antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in Neuro-2a cells. Anti- c-Rel antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).