PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
应用 | 稀释度 |
---|---|
FCM | 1:2000 |
Ly-6G protein is a GPI (glycosylphosphatidylinositol)-anchored protein. Ly-6G is primarily expressed on monocytes, neutrophils, and T cells. It plays a crucial role in inhibiting anti-tumor efficacy and T-cell responses. Due to its specific expression on neutrophils, Ly-6G has been widely used as a surface marker in flow cytometry, cell sorting, and immune phenotyping experiments. Unique expression of Ly-6G in bone marrow indicates its involvement in hematopoietic microenvironment, potentially regulating the development or function of immune cells within the bone marrow niche.
Flow cytometric analysis of C57BL/6 mouse bone marrow labeling mouse Ly-6G at 1/2000 (0.1 μg) dilution / (Right panel) compared with a Rat IgG, Isotype Control / (Left panel). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD11b - Brilliant Violet 510 separately. Gated on myeloid cells.