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Phospho-Histone H3 (Ser10) Rabbit Polyclonal Antibody

Phospho-Histone H3 (Ser10) Rabbit Polyclonal Antibody

货号: S0B0751
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-Histone H3 (Ser10)

  • 分子别名

    H3S10P

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 抗体同种型

    IgG

  • 应用

    ChIP, WB

  • 反应种属 ?

    Hu

  • 预测反应种属
    (反应种属缩写表)

    Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe

  • 纯化方式

    Immunogen Affinity

  • 浓度

    4 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
Dot Blot 1:1000
WB 1:1000
ChIP 1:20-1:50
背景介绍

  • H3S10P is an epigenetic modification to the DNA packaging protein histone H3. It is a mark that indicates the phosphorylation the 10th serine residue of the histone H3 protein. The S10 phosphorylation is involved in mitosis, transcription, chromatin condensation, and UVB response. H3S10p causes chromosome condensation and segregation during cell mitosis. H3S10p temporarily increases during mitosis while H3K9me3 decareses and H3K9me3 recovers upon mitotic exit.

  • 免疫印迹

    • WB result of Phospho-Histone H3 (Ser10) Rabbit pAb
      Primary antibody: Phospho-Histone H3 (Ser10) Rabbit pAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 100 ng/ml Nocodazole for 18 hours, then treated with 100 nM Calyculin A for 1 hr whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

  • 免疫沉淀


  • 斑点杂交

    • Dot blot result of Phospho-Histone H3 (Ser10) Rabbit pAb
      Lane1: Histone H3 (Ser10) phospho peptide
      Lane2: Histone H3 (Ser10) phospho peptide
      Lane3: Histone H3 non-phospho peptide
      Primary antibody: Phospho-Histone H3 (Ser10) Rabbit pAb at 1/1000 dilution
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • ChIP

    • Chromatin immunoprecipitation (ChIP) was performed on HeLa + Nocodazole (100ng/ml,18h) (+) (upper panel) and HeLa + Nocodazole (100ng/ml,18h) (-) (lower panel) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented bysonication.
      Parallel reactions used Phospho-Histone H3 (Ser10) Rabbit Polyclonal Antibody and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
      Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
      qPCR (%input: immunoprecipitated DNA/input DNA)
      showed the enrichment of RPL30 and GAPDH in Phospho-Histone H3
      (Ser10) Rabbit Polyclonal Antibody -immunoprecipitated sample.