您现在的位置: 首页   -  产品中心   -  免疫学   -   抗体
PRMT1 Recombinant Rabbit mAb (S-884-10)

PRMT1 Recombinant Rabbit mAb (S-884-10)

货号: S0B0752
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    PRMT1
  • 分子别名

    Protein arginine N-methyltransferase 1, Histone-arginine N-methyltransferase PRMT1, Interferon receptor 1-bound protein 4, HMT2, HRMT1L2, IR1B4
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm, Nucleus
  • Accession

    Q99873
  • 克隆号

    S-884-10
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    ICFCM, IHC-P, ICC, WB, IP
  • 反应种属 ?

    Hu, Ms, Rt
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:250
ICC 1:500
ICFCM 1:500
IP 1:50
背景介绍
  • PRMT1 protein, or Protein Arginine Methyltransferase 1, is a key enzyme in cellular biology with diverse functions. PRMT1 catalyzes the symmetric dimethylation of arginine residues in proteins, affecting the structure and function of these proteins. It is involved in multiple biological processes, including cell differentiation, RNA processing, nucleolar formation, and gene transcription and post-transcriptional modification. PRMT1 has been linked to the regulation of miRNA biogenesis by methylating miRNA synthesis-related proteins. PRMT1 is implicated in a wide range of diseases due to its abnormal expression or dysfunction. It has been associated with leukemia, tumors, cardiovascular diseases, and multiple cancer types, including breast cancer, lung cancer, colon cancer, bladder cancer, and blood cancer.

  • 免疫印迹

    • WB result of PRMT1 Recombinant Rabbit mAb
      Primary antibody: PRMT1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HepG2 whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 38 kDa

    • WB result of PRMT1 Recombinant Rabbit mAb
      Primary antibody: PRMT1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 38 kDa

    • WB result of PRMT1 Recombinant Rabbit mAb
      Primary antibody: PRMT1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 38 kDa
      This blot was developed with high sensitivity substrate

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) labelling PRMT1 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • PRMT1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating PRMT1 in 0.4 mg HepG2 whole cell lysate.
      Western blot was performed on the immunoprecipitate using PRMT1 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HepG2 whole cell lysate 20 µg (Input)
      Lane 2: PRMT1 Rabbit mAb IP in HepG2 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
      Predicted MW: 42 kDa
      Observed MW: 38 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human testis. Anti-PRMT1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human cardiac muscle. Anti-PRMT1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PRMT1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PRMT1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon. Anti-PRMT1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HepG2 cells. Anti-PRMT1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).