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KDM1B Recombinant Rabbit mAb (S-993-8)

KDM1B Recombinant Rabbit mAb (S-993-8)

货号: S0B0746
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    Lysine-specific histone demethylase 2, Flavin-containing amine oxidase domain-containing protein 1, Lysine-specific histone demethylase 1B, AOF1, C6orf193, LSD2

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    Q8NB78

  • 克隆号

    S-993-8

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICFCM, ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
ICC 1:400-1:2000
ICFCM 1:200
背景介绍

  • KDM1B, also known as LSD2 or Aof1, is an enzyme that plays crucial roles in various biological processes. It has FAD-binding activity and FAD-dependent demethylase activity specific to histone H3-di/monomethyl-lysine-4. It is involved in the regulation of DNA methylation, particularly upstream of spermatogenesis, histone H3-K4 demethylation, and the regulation of gene expression by genetic imprinting.

  • 免疫印迹

    • WB result of KDM1B Recombinant Rabbit mAb
      Primary antibody: KDM1B Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HepG2 whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: HCT 116 whole cell lysate 20 µg
      Weak expression: HepG2 whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 92 kDa
      Observed MW: 92 kDa
      This blot was developed with high sensitivity substrate

    • WB result of KDM1B Recombinant Rabbit mAb
      Primary antibody: KDM1B Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: mouse thymus lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 92 kDa
      Observed MW: 92 kDa

    • WB result of KDM1B Recombinant Rabbit mAb
      Primary antibody: KDM1B Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: rat thymus lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 92 kDa
      Observed MW: 92 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling KDM1B antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-KDM1B antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).