PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| ICFCM | 1:2000 |
mCherry is a widely used red fluorescent protein in biotechnology, exhibiting several key properties and applications. mCherry originates from the coral Discosoma species and serves as a monomeric red fluorescent protein. It absorbs light in the range of 540-590 nm and emits light in the range of 550-650 nm. Unlike GFP (Green Fluorescent Protein) and its variants derived from jellyfish, mCherry possesses distinct red fluorescent properties. It is commonly used as a red fluorescent tag protein for labeling and localizing molecular components within cells.
WB result of mCherry Mouse mAb
Primary antibody: mCherry Mouse mAb at 1/1000 dilution
Lane 1: untreated 293T whole cell lysate 20 µg
Lane 2: 293T transfected with Histone H3-mCherry-E-tag whole cell Lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 48 kDa
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Histone H3-mCherry-E-Tag transfected 293T (Human embryonic kidney epithelial cell) labelling CBP Tag antibody at 1/2000 (0.1 μg) dilution/ (Right panel) compared with a Mouse IgG Isotype Control / (Left panel). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
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