PBS pH7.4
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
Sandwich ELISA assay
Coating HER2 recombinant protein: 5μg/ml
Sample: Trastuzumab deruxtecan (DXD ADC), from 6.25ng/ml, 2-fold diluent, 6 points
Add Biotin conjugated anti-Dxd/Exatecan Rabbit mAb (S-R418), 0.5μg/ml
Add SA-HRP, 1:20000
ELISA analysis specificity of Dxd/Exatecan rabbit mAb
Coating ADC: 1μg/ml;
Add anti-Dxd/Exatecan Recombinant Rabbit mAb (biotin conjugated): from 1μg/ml, 3-fold diluent, 7 points;
Add SA-HRP: 1:10000