PBS, 0.1% BSA, 0.01% Proclin 300
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
Flow cytometric analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) labeling SDT Alexa Fluor® 488 Mouse IgG1, κ Isotype Control Antibody at 1 μg/test (Left) / (Red line histogram) compared with SDT CD19 antibody at 1 μg/test (S0B2311) (Right) / (Red line histogram), Mouse monoclonal IgG Isotype Control (Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody) (Left) / (Black line histogram) compared with SDT Alexa Fluor® 488 Mouse IgG1, κ Isotype Control Antibody (Right) / (Black line histogram) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Black line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Mouse embryonic fibroblast) labelling SDT Alexa Fluor® 488 Mouse IgG1, κ Isotype Control Antibody at 1 μg/test (Left) / (Red line histogram) compared with α-tubulin antibody (Right) / (Red line histogram), Mouse monoclonal IgG Isotype Control (Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody) (Left) / (Black line histogram) compared with SDT Alexa Fluor® 488 Mouse IgG1, κ Isotype Control Antibody (Right) / (Black line histogram) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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