Ser79-Gln267 with His tag and TEV site at N-Terminus
20 mM Tris, pH 8.0, 150 mM NaCl.
·6 months from date of receipt, -60 to -80 °C as supplied.
·about 3 days in 2 to 8 °C.
·Please avoid repeated freeze-thaw cycles.
1. Grinkova Y V, Denisov I G, Sligar S G. Engineering extended membrane scaffold proteins for self-assembly of soluble nanoscale lipid bilayers[J]. Protein engineering, design & selection, 2010, 23(11): 843-848.
The soluble lipid bilayer nanodisc has proven to be a widely applicable method for membrane proteins as soluble in aqueous solutions, which can monodisperse and active membrane protein in a closely native bilayer environment. The key component of the nanodisc is the surrounding amphiphilic spiral protein band (membrane scaffold protein). Nanodisc systems have been used to reconstruction a variety of proteins including GPCRs, P450, bacterial rhodopsin, clotting factors, and tar receptors. Membrane scaffold protein 1D1 has been used as a scaffold protein for stabilizing lipid nanodiscs (ND). Membrane scaffold protein 1D1 (MSP1D1) is modified versions of apolipoprotein A1. It is a biparental synthetic protein that can self-assemble to form nanodiscs. The diameter of Nanodiscs is about 9.7 nm.
A is MSP1D1 His protein; B is POPC lipid; C is mix of MSP1D1 His protein and Lipids; The activity of MSP1D1 His protein was performed by reconstructed with lipids. The peak volume of empty MSP1D1 in HPLC is about 14.9 ml, but the peak volume of MSP1D1 reconstructed lipids is about 12.05 ml, which shows that the MSP1D1 have reconstruction function.
2μg (R: reducing condition, N: non-reducing condition).
The purity of MSP Protein his tag thrombin site in detergent is greater than 95% as determined by SEC-HPLC.
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