A2-V672 end,with N-GST-TEV
50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 5% glycerol, 5 mM DTT, 0.1 M Trehalose
-80℃
The activity of PKCα is verified by the ADP-Glo method: Incubate PKCα protein, ATP, and the
substrate at room temperature for 40 minutes, then add the ADP-Glo reagent and read the
luminescence signal using the chemiluminescence module of the microplate reader.