M1-K458 (end), with N-terminal 8x His and Flag tag
50 mM Tris, 150 mM NaCl, 1mM DTT, 10% glycerol, pH7.5
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
The KAT8 activity was detected using homogeneous time-resolved fluorescence technology. The reaction was performed by incubating the KAT8 protein and substrate at 25℃ for 90 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 nm with BMG.
M MW Standard
1 1 μg KAT8
2 3 μg KAT8
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