R162-L513(end), with N-terminal GST tag
66.3 kDa
50 mM Tris, 150 mM NaCl, 20 mM GSH, 1 mM DTT, 10% glycerol, pH7.5
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
The ACVR2A activity was detected using HTRF technology. The reaction was performed by incubating the ACVR2A protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
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