A2-P379 end, N-GST-TEV
69.3 KDa
50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT
-80℃
Please use rapid thawing with running water to thaw the protein samples.
For complete recovery, mix well and spin before use.
Product must not be stored in diluted solutions.
Aliquots below 10μl are not advisable.
Avoid repetitive freeze-thaw cycles!
The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with
fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were
separated and detected by MSA device. The conversion rate of ERK1 is 30% when it reacts with 20 μM ATP
and 2 μM Peptide 8 for 30 minutes, and the corresponding enzyme concentration is 25 nM.
Lane M: Marker
Lane 1: 2 μg, reduced
Lane 2: 2 μg, non-reduced
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