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Nanobody-eGFP-002 Protein

Nanobody-eGFP-002 Protein

货号: UA060003
价格: 2480
规格: 25μg
介绍: -
其他: -
产品规格

  • 物种

    Vicugna pacos

  • 分子别名

    Nanobody

  • Accession

    NCBI PUBMED ID: 31562305

  • 表达序列

    Wild type with His tag-3C site-eGFP at N-Terminus

  • 分子量

    43.1 kDa

  • 纯度

    ＞95% by SDS-PAGE &＞95% by SEC-HPLC

  • 内毒素含量

    <0.1EU/μg

  • 活性

    The function of Nanobody-eGFP protein is track expression of membrane protein with different designs by FSEC platform in HEK293 or insect systems; Firstly, the membrane protein with alfa tag was expressed in HEK293 or insect systems, and soluble the cells with detergent, and the supernatant after soluble and centrifuged was incubated with nanobody-eGFP, then the mix protein samples after incubate was inject to HPLC-FSEC platform; The fluorescence value of sample would be screened the various design of membrane protein, and help us to select the high expression construct for next research.

  • 标记

    Unconjugated

  • 标签蛋白&酶切位点

    No

  • 性状

    Lyophilized Powder

  • 缓冲体系

    35mM PB, 1M NaCl, pH 7.4.

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·6 months from date of receipt, -60 to -80 °C as supplied.
    ·about 3 days in 2 to 8 °C.
    ·Please avoid repeated freeze-thaw cycles.

  • 稀释度

  • 生物活性JSON

    • Figure A is nanobody-eGFP as control. Figure B and C is final sample of membrane protein with saturation and non-saturation compare to nanobody-eGFP. Figure D and E is supernatant sample of membrane protein with saturation and non-saturation. From the five pictures, the results indicated that the nanobody-eGFP have high binding activity to membrane protein-alfa tag.

  • 电泳JSON

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱JSON(SEC-HPLC)

    • 91.1%.