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Biotinylated Nectin-4 His & Avi Tag Protein, Mouse

Biotinylated Nectin-4 His & Avi Tag Protein, Mouse

货号: UA011154
价格: 2800
规格: 25μg
介绍: -
其他: -
产品规格
  • 物种

    Mouse
  • 分子别名

    NECTIN4, LNIR, PRR4, PVRL4
  • Accession

    Q8R007-1
  • 表达序列

    Gly31-Ser347 with His Tag & Avi Tag at C-Terminus

  • 表达宿主

    HEK293
  • 分子量

    43-47kDa (Reducing)
  • 纯度

    >95% by SDS-PAGE & >90% by SEC-HPLC
  • 活性

    Immobilized Biotinylated Nectin-4 His & Avi Tag Protein, Mouse (Cat. No. UA011154) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind Anti-Human Nectin-4 (Enfortumab) with EC50 of 3.55-5.02 ng/ml.
  • 标记

    Biotin
  • 标签

    Avi Tag, His Tag
  • 性状

    Lyophilized Powder
  • 缓冲体系

    PBS, PH7.4, 5% trehalose
  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. 
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.  
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Duan X, Zhang Z, Xu H, Zhang J, Yan Y, Yang X. Preclinical Evaluation of an Al18F-Radiolabeled Bicyclic Peptide Targeting Nectin-4. Mol Pharm. 2024 Dec 9.

  • 稀释度

背景介绍
  • Nectin-4, a member of the Nectin and nectin-like family of cell adhesion molecules, is highly expressed in normal embryonic and fetal tissues, with expression levels decreasing after reaching adulthood and being restricted in healthy adult tissues. Nectin-4 is overexpressed in various types of tumor cells and is known to promote tumor cell growth and proliferation. Solid tumors that frequently exhibit high Nectin-4 expression include bladder, pancreatic, triple-negative breast, non-small cell lung, stomach, esophageal, and ovarian cancers, among others.
    Research has shown that Nectin-4 enhances tumor cell proliferation, differentiation, migration, and invasion by activating the PI3K/Akt signaling pathway, suggesting a significant role in cancer initiation and metastasis. These findings have prompted the development of drugs targeting Nectin-4 as a potential therapeutic strategy.

  • 电泳JSON

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱JSON(SEC-HPLC)

    • The purity of Biotinylated Nectin-4 His&Avi Tag Protein, Mouse is more than 90% determined by SEC-HPLC.