M109-Q496(end), with N-terminal 6x His tag
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
The NMT1 activity was detected by FI assay. The reaction was performed by incubating the NMT1 protein, CMP and myristoylcon, adding HSPP60SRC at 25℃ for 60 min, then reading ratio 380/470 with BMG.
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