Ile140-Asp317
21 kDa (Reducing)
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.
Cell. 2000 Sep 29;103(1):41-50.
Genes Dev. 1999 Sep 15;13(18):2412-24.
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as TNF-related activation-induced cytokine (TRANCE), is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily. In humans, RANKL is primarily expressed by osteoblasts, T cells, and other immune cells, and plays a crucial role in regulating bone metabolism, immune function, and the development of mammary glands. RANKL functions by binding to its receptor RANK on the surface of osteoclast precursors, leading to the activation and differentiation of osteoclasts, which are responsible for bone resorption. Additionally, RANKL is involved in the regulation of T cell activation, dendritic cell function, and the formation of lactating mammary glands. Dysregulation of RANKL has been implicated in various bone-related diseases, such as osteoporosis and bone metastasis, as well as in immune disorders, highlighting the importance of RANKL in skeletal biology and immune regulation.
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The EC50 for this effect is less than 1ng/ml.
2μg (R: reducing condition, N: non-reducing condition).
>95% as determined by SEC-HPLC.