R669-A1210(end), C797S with N-terminal GST tag
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
The EGFR[C797S] activity was detected using HTRF technology. The reaction was performed by incubating the EGFR[C797S] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.