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ALCAM/CD166 His Tag Protein, Human

ALCAM/CD166 His Tag Protein, Human

货号: UA011112
价格: 600
规格: 25μg
介绍: -
其他: -
产品规格

  • 物种

    Human

  • 分子别名

    CD166 antigen，Activated leukocyte cell adhesion molecule，CD166，ALCAM，MEMD，Activated Leucocyte Cell Adhesion Molecule，Activated-Leukocyte Cell Adhesion Molecule

  • Accession

    Q13740-1

  • 表达序列

    Trp28-Ala526 with His Tag at C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    70-90kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE &＞90% by SEC-HPLC

  • 内毒素含量

    <0.1EU/μg

  • 活性

    Immobilized ALCAM/CD166 His Tag Protein, Human (Cat. No. UA011112) at 5 μg/mL (100μL/well) can bind Human CD6, hFc tag (Cat. No. S0A1088) with EC50 of 0.14-0.18 μg/mL.

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, PH7.4, 5% trehalose

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Swart GW. (2002) Activated leukocyte cell adhesion molecule (CD166/ALCAM): developmental and mechanistic aspects of cell clustering and cell migration. Eur J Cell Biol. 81(6): 313-21.

  • 稀释度

背景介绍

  • Activated Leukocyte Cell Adhesion Molecule (ALCAM), also known as Cluster of Differentiation 166 (CD166), is a type I transmembrane cell adhesion molecule that belongs to the immunoglobulin (Ig) superfamily. It serves as a ligand for CD6 and is expressed on T lymphocytes. The interaction between ALCAM and CD6 is crucial for T cell development, regulation, and for the binding of T- and B-cells to activated leukocytes.
    ALCAM is expressed in a variety of tissues but is typically restricted to cell subsets involved in dynamic growth and/or migration. This includes processes such as neural development, branching organ development, hematopoiesis, immune response, and tumor progression. High expression levels of ALCAM on melanoma cell lines seem to promote metastasis, whereas in breast cancer, it has been associated with anti-metastatic activity.
    ALCAM may also influence the expression or adhesion of the neuronal adhesion molecule NCAM-L1, as observed in both the developing retina and invasive melanoma.

  • 电泳JSON

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱JSON(SEC-HPLC)

    • The purity of ALCAM/CD166 His Tag Protein, Human is greater than 90% as determined by SEC-HPLC.

  • ELISA

    • Immobilized ALCAM/CD166 His Tag Protein, Human (Cat. No. UA011112) at 5 μg/mL (100μL/well) can bind Human CD6, hFc tag (Cat. No. S0A1088) with EC50 of 0.14-0.18 μg/mL.