12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| ICC | 1:100 |
| ICFCM | 1:50 |
Ribonucleotide reductase large subunit M1 (RRM1) is a rate-limiting enzyme of the DNA synthesis pathway, and is depended on for the conversion of ribonucleotides to dNTPs. Studies have shown that increased levels of RRM1 are associated with resistance to gemcitabine. RRM1 has also been reported to affect disease prognosis of pancreatic cancer. In fact, the meta-analysis for RRM1 demonstrated that high expression of RRM1 was significantly associated with worse overall survival. The clinical impact of RRM1 has been elucidated
WB result of RRM1 Rabbit mAb
Primary antibody: RRM1 Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
WB result of RRM1 Rabbit mAb
Primary antibody: RRM1 Rabbit mAb at 1/1000 dilution
Lane 1: RAW 264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
WB result of RRM1 Rabbit mAb
Primary antibody: RRM1 Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labelling RRM1 antibody at 1/50 (1 μg) dilution / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
ICC shows positive staining in HT-29 cells. Anti-RRM1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
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