E636-D1331,with N-terminal His-Avi tag
85.2 kDa
50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
The Biotin-BRM activity was quantified using a SPR-based assay. The Biotin-BRM protein was immobilized onto the SA chip using labeled biotin. The varying concentration of FHD-286 was injected and flowed over onto the Biotin-BRM protein surface. And the response signal was recorded real time. The kinetics fitting was as follows with a kD value of ~ 4.29x10-9 M.
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